Survey of Anaplasmataceae bacteria in sheep from Senegal [texte imprimé] / Mamadou Lamine Djiba, Auteur ; Oleg Mediannikov, Auteur ; M. Mbengue, Auteur ; Y Thiongane, Auteur ; Jean-François Molez, Auteur ; Mor Talla Seck, Auteur ; Florence Fenollar, Auteur ; Didier Raoult, Auteur ; M Ndiaye, Auteur . - Dakar (PRH, Sénégal) : ISRA/LNERV, 2013 . - 05p. : ref,;. Langues : Anglais ( eng) Catégories : | SCIENCES, PRODUCTION ET PROTECTION ANIMALES
| Mots-clés : | Mouton, Anaplasmosis, Bactérie, Sénégal, Maladie des Ruminants | Index. décimale : | L723-Bactéries des ruminants, biologies des bacteries | Résumé : | Purpose The authors studied the role of bacteria belonging to Anaplasmataceae family as the causes of acute illnesses of sheep in West Africa.
Methods We examined and sampled 120 febrile sheep in two regions of Senegal for this study. The DNA extracted from these blood samples was tested by PCR using two pairs of primers (groEL-based and 16S rRNA gene-based). Results In 52/120 samples, the microscopic examination revealed intraerythrocytic and/or intraphagocytic spherical inclusions. In 48/52 cases, we succeeded in identifying the bacterial agent: in 38 cases, it was Anaplasma ovis; in six cases, it was Ehrlichia ruminantium; in two cases, Anaplasma phagocytophilum; in one case, Anaplasma platys; and in one
case, a yet uncultured Anaplasma sp. closely related to A. phagocytophilum. Conclusions Our studies demonstrated the great variety of pathogenic bacteria from the Anaplasmataceae family in the blood of clinically ill sheep. A. ovis was identified unexpectedly often. For the first time, A. phagocytophilum was found in sub-Saharan Africa, and its further epidemiology may be now reconsidered. The roles of canine pathogen, A. platys, and yet undescribed Anaplasma sp. “Badiouré” in ovine pathology should be more closely studied. |
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