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Auteur Didier Raoult
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Affiner la rechercheGenomic analysis of three African strains of Bacillus anthracis demonstrates that they are part of the clonal expansion of an exclusively pathogenic bacterium / L. Rouli
Genomic analysis of three African strains of Bacillus anthracis demonstrates that they are part of the clonal expansion of an exclusively pathogenic bacterium [texte imprimé] / L. Rouli, Auteur ; M. Mbengue, Auteur ; C. Robert, Auteur ; M. Ndiaye, Auteur ; B. La Scola, Auteur ; Didier Raoult, Auteur . - Dakar (PRH, Sénégal) : ISRA/LNERV, 2014 . - 09p.
Langues : Anglais (eng)
Catégories : SCIENCES, PRODUCTION ET PROTECTION ANIMALES Mots-clés : Bacillus Anthracis, Race Africaine, Génome, Bacterium Pathogénique Index. décimale : L733-Maladie bactériennes des ruminants. Symptomologie et dégats Résumé : Bacillus anthracis is the causative agent of anthrax and is classified as a ‘Category A’ biological weapon. Six complete genomes of B. anthracis
(A0248, Ames, Ames Ancestor, CDC684, H0491, and Sterne) are currently available. In this report, we add three African strain genomes:
Sen2Col2, Sen3 and Gmb1. To study the pan-genome of B. anthracis, we used bioinformatics tools, such as Cluster of Orthologous Groups,
and performed phylogenetic analysis. We found that the three African strains contained the pX01 and pX02 plasmids, the nonsense
mutation in the plcR gene and the four known prophages. These strains are most similar to the CDC684 strain and belong to the A cluster.
We estimated that the B. anthracis pan-genome has 2893 core genes (99% of the genome size) and 85 accessory genes. We validated the
hypothesis that B. anthracis has a closed pan-genome and found that the three African strains carry the two plasmids associated with
bacterial virulence. The pan-genome nature of B. anthracis confirms its lack of exchange (similar to Clostridium tetani) and supports its
exclusively pathogenic role, despite its survival in the environment. Moreover, thanks to the study of the core content single nucleotide
polymorphisms, we can see that our three African strains diverged very recently from the other B. anthracis strains.Exemplaires
Code-barres Cote Support Localisation Section Disponibilité ZV0170105 L733- ROU Article scientifique LNERV Rayons Exclu du prêt Documents numériques
Cliquer pour voir pdfURL Survey of Anaplasmataceae bacteria in sheep from Senegal / Mamadou Lamine Djiba
Survey of Anaplasmataceae bacteria in sheep from Senegal [texte imprimé] / Mamadou Lamine Djiba, Auteur ; Oleg Mediannikov, Auteur ; M. Mbengue, Auteur ; Y Thiongane, Auteur ; Jean-François Molez, Auteur ; Mor Talla Seck, Auteur ; Florence Fenollar, Auteur ; Didier Raoult, Auteur ; M Ndiaye, Auteur . - Dakar (PRH, Sénégal) : ISRA/LNERV, 2013 . - 05p. : ref,;.
Langues : Anglais (eng)
Catégories : SCIENCES, PRODUCTION ET PROTECTION ANIMALES Mots-clés : Mouton, Anaplasmosis, Bactérie, Sénégal, Maladie des Ruminants Index. décimale : L723-Bactéries des ruminants, biologies des bacteries Résumé : Purpose The authors studied the role of bacteria belonging to Anaplasmataceae family as the causes of acute illnesses of sheep in West Africa.
Methods We examined and sampled 120 febrile sheep in two regions of Senegal for this study. The DNA extracted from these blood samples was tested by PCR using two pairs of primers (groEL-based and 16S rRNA gene-based). Results In 52/120 samples, the microscopic examination revealed intraerythrocytic and/or intraphagocytic spherical inclusions. In 48/52 cases, we succeeded in identifying the bacterial agent: in 38 cases, it was Anaplasma ovis; in six cases, it was Ehrlichia ruminantium; in two cases, Anaplasma phagocytophilum; in one case, Anaplasma platys; and in one
case, a yet uncultured Anaplasma sp. closely related to A. phagocytophilum. Conclusions Our studies demonstrated the great variety of pathogenic bacteria from the Anaplasmataceae family in the blood of clinically ill sheep. A. ovis was identified unexpectedly often. For the first time, A. phagocytophilum was found in sub-Saharan Africa, and its further epidemiology may be now reconsidered. The roles of canine pathogen, A. platys, and yet undescribed Anaplasma sp. “Badiouré” in ovine pathology should be more closely studied.Exemplaires
Code-barres Cote Support Localisation Section Disponibilité ZV0170041 L723- DJI Article scientifique LNERV Rayons Exclu du prêt Documents numériques
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Cliquer pour voir pdfURL Fiches Techniques ISRA, Vol. 8, N°2. Bactéries de la famille des anaplasmataceae dans deux zones écologiques (Niayes et Ziguinchor) du Sénégal / Mamadou Lamine Djiba
Fiches Techniques ISRA, Vol. 8, N°2. Bactéries de la famille des anaplasmataceae dans deux zones écologiques (Niayes et Ziguinchor) du Sénégal [texte imprimé] / Mamadou Lamine Djiba, Auteur ; Oleg Mediannikov, Auteur ; Mbaye Mbengue, Auteur ; Yaya Thiongane, Auteur ; Jean-François Molez, Auteur ; Mor Talla Seck, Auteur ; Florence Fenollar, Auteur ; Didier Raoult, Auteur ; Mady Ndiaye, Auteur . - 2015 . - 9 p.
Langues : Français (fre)
Catégories : SCIENCES, PRODUCTION ET PROTECTION ANIMALES Index. décimale : L733-Maladie bactériennes des ruminants. Symptomologie et dégats Exemplaires
Code-barres Cote Support Localisation Section Disponibilité aucun exemplaire New Microbes and New Infections. Genomic analysis of three African strains of Bacillus anthracis demonstrates that they are part of the clonal expansion of an exclusively pathogenic bacterium / L. Rouli
New Microbes and New Infections. Genomic analysis of three African strains of Bacillus anthracis demonstrates that they are part of the clonal expansion of an exclusively pathogenic bacterium [texte imprimé] / L. Rouli, Auteur ; Mbaye Mbengue, Auteur ; C. Robert, Auteur ; M Ndiaye, Auteur ; B. La Scola, Auteur ; Didier Raoult, Auteur . - (BE) : John Wiley & Sons Ltd, 2014 . - 9 p.
Langues : Anglais (eng)
Catégories : SCIENCES, PRODUCTION ET PROTECTION ANIMALES Mots-clés : Souche africaine, Bacillus anthracis, pan-génome, pathogène Index. décimale : L730-Maladie des animaux Résumé : Bacillus anthracis is the causative agent of anthrax and is classified as a ‘Category A’ biological weapon. Six complete genomes of B. anthracis
(A0248, Ames, Ames Ancestor, CDC684, H0491, and Sterne) are currently available. In this report, we add three African strain genomes:
Sen2Col2, Sen3 and Gmb1. To study the pan-genome of B. anthracis, we used bioinformatics tools, such as Cluster of Orthologous Groups,
and performed phylogenetic analysis. We found that the three African strains contained the pX01 and pX02 plasmids, the nonsense
mutation in the plcR gene and the four known prophages. These strains are most similar to the CDC684 strain and belong to the A cluster.
We estimated that the B. anthracis pan-genome has 2893 core genes (99% of the genome size) and 85 accessory genes. We validated the
hypothesis that B. anthracis has a closed pan-genome and found that the three African strains carry the two plasmids associated with
bacterial virulence. The pan-genome nature of B. anthracis confirms its lack of exchange (similar to Clostridium tetani) and supports its
exclusively pathogenic role, despite its survival in the environment. Moreover, thanks to the study of the core content single nucleotide
polymorphisms, we can see that our three African strains diverged very recently from the other B. anthracis strains.Exemplaires
Code-barres Cote Support Localisation Section Disponibilité ZV0170105.pdf L730-ROU Article scientifique LNERV Rayons Exclu du prêt Documents numériques
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