AURIFERE
PORTAIL DOCUMENTAIRE DE L'INSTITUT SENEGALAIS DE RECHERCHES AGRICOLES
Elaboré par
L’Unité d’Information et de Valorisation des Résultats de la Recherche (UNIVAL)
'Un Accès Unique aux Ressources d’Information
et aux Fonds documentaires pour une Recherche d’Excellence
'
A partir de cette page vous pouvez :
Détail de l'auteur
Documents disponibles écrits par cet auteur
Affiner la recherche
Journal of Applied Microbiology,, N°108:. Characterization of Mesorhizobiumspp. strains associated with Acacia seyal Del. in Senegal by phenotypic, symbiotic, and molecular methods [texte imprimé] / D. Diouf, Auteur ; D Fall, Auteur ; C. Chaintreuil, Auteur ; A.T. Bâ, Auteur ; B. Dreyfus, Auteur ; M. Neyra, Auteur ; I. Ndoye, Auteur ; L Moulin, Auteur . - 2010 . - pp - 818-830. Langues : Anglais ( eng) Catégories : | FORESTERIE
| Résumé : | To assess the phenotypic, symbiotic and genotypic diversity scope of Mesorhizobium spp. strains associated with Acacia seyal (Del.) isolated from different agro-ecological zones in Senegal, and uses of susceptible microbial inoculum in a reafforestation process. A polyphasic approach including phenotypic and genotypic techniques was used to study the diversity and their relationships with other biovars and species of rhizobia. The geographical origins of the strains have limited effect on their phylogenetic and phenotypic classification. Nodulation tests indicated promiscuity of the strains studied, because they were capable of nodulating six woody legume species (Acacia auriculiformis, Acacia senegal, A. seyal, Acacia tortilis ssp. raddiana, Leucaena leucocephala and Prosopis juliflora). Sequencing and phylogenetic analyses of nodA, nodC and nifH genes pointed out that in contrast to nodA gene, the phylogenies of nodC and nifH genes were not consistent with that of 16S rRNA, indicating that these genes of the A. seyal-nodulating rhizobia might have different origins. Microbial inoculation on nonsterile soil had significant effect on the nodules number and the growth of the seedlings, indicating that these strains of rhizobia might be used as inoculum. The results indicated that A. seyal is a nonselective host that can establish effective symbiosis with Mesorhizobium spp. strains from diverse genomic backgrounds and that the selected A. seyal-nodulating rhizobia could enhance plant growth. These results showed the important role that A. seyal could play in the improvement of reafforestation process as a promiscuous host, which can establish effective symbiosis with rhizobia from diverse genomic backgrounds. |
|
Exemplaires
Disponibilité |
---|
FT1900062 | DIO | Publication | CNRF | Rayons | Exclu du prêt |
Documents numériques
Cliquer pour voirURL | | |
PLOS ONE, In press. Genetic and GenomicDiversityStudies of AcaciaSymbionts in Senegal Reveal New Species of Mesorhizobiumwith a Putative Geographical Pattern [texte imprimé] / F Diouf, Auteur ; D. Diouf, Auteur ; A Klonowska, Auteur ; A Le Queré, Auteur ; N Bakhoum, Auteur ; D Fall, Auteur ; M Neyra, Auteur ; H Parrinello, Auteur ; I. Ndoye, Auteur ; L Moulin, Auteur . - 2015. Langues : Anglais ( eng) Catégories : | FORESTERIE
| Mots-clés : | Acacia Sénégal | Index. décimale : | K30-Génétique et amélioration des plantes forestiéres | Résumé : | Acacia senegal (L) Willd. and Acacia seyal Del. are highly nitrogen-fixing and moderately salt tolerant species. In this study we focused on the genetic and genomic diversity of Acacia mesorhizobia symbionts from diverse origins in Senegal and investigated possible correla- tions between the genetic diversity of the strains, their soil of origin, and their tolerance to sa- linity. We first performed a multi-locus sequence analysis on five markers gene fragments on a collection of 47 mesorhizobia strains of A. senegal and A. seyal from 8 localities. Most of the strains (60%) clustered with the M. plurifarium type strain ORS 1032T, while the others form four new clades (MSP1 to MSP4). We sequenced and assembled seven draft genomes: four in the M. plurifarium clade (ORS3356, ORS3365, STM8773 and ORS1032T), one in MSP1 (STM8789), MSP2 (ORS3359) and MSP3 (ORS3324). The average nucleotide identities be- tween these genomes together with the MLSA analysis reveal three new species of Mesorhi- zobium. A great variability of salt tolerance was found among the strains with a lack of correlation between the genetic diversity of mesorhizobia, their salt tolerance and the soils samples characteristics. A putative geographical pattern of A. senegal symbionts between the dryland north part and the center of Senegal was found, reflecting adaptations to specific local conditions such as the water regime. However, the presence of salt does not seem to be an important structuring factor of Mesorhizobium species. |
|
Exemplaires
Disponibilité |
---|
FT1900047 | K30- DIO | Publication | CNRF | Rayons | Exclu du prêt |
Documents numériques
Cliquer pour voirURL | | |