yle--+-- REPUBLIQUE DU SENEGAL ----- MINISTERE...
yle--+--
REPUBLIQUE DU SENEGAL
-----
MINISTERE DU DEVELOPPEMENT
RURAL ET DE L'HYDRAULIQUE
-----
INSTITUT SENEGALAIS DE RJXHERCHES
AGRICOLES (1-S-R-A.)
-w--m
DIRECTION DES RECHERCHES SUR LES
5-Y
PRODUCTIONS ET LA SANTJZ ANIMALES
y!/,
--e-w
G
LABORATOIRE NATIONAL DE L'ELEVAGE
ET DE RECHl$RCHES VETERINAIRES
DAKAR/HANN
ANIMAL PASTEURELLOSIS
The Senegalese experience
Dr. Mamady KONTE
Paper presented at the "International
REF, No 12 /PATHo. ANIM.
Workshop en Pasteurellosis in Animal
AVRIL 1992
Production", Bali, Indonesia,
loth - 13th 1992.
/
ANIMAL PASTEURELLOSIS :
THE SENEGALESE EXPERIENCE.
INTRODUCTION
- m a r k e d interest i n pasteurellcsis
since 1930.
- p r o d u c t i o n o f p o u l t r y ,
cat.t!e and sheep vaccines despi te
the u s e o f unsuited b u i l d i n g .
- i n t e n s i f i c a t i o n o f
diagnosis
activit.ies
and
vacc’i ne
p r o d u c t i o n aft.er m o v i n g i n s u i t e d b u i l d i n g .
organization
and
i n t e n s i f i c a t i o n o f
r e s e a r c h o n
epidemiology.
- b r i g h t e r f u t u r e
a n d prospects f o r r e s e a r c h o n a n i m a l
pathologies
a f t e r a c q u i s i t i o n o f m o d e r n f e r m e n t o r .
PASTEURELLOSIS BEFORE THE USE OF FERMENTOR
A2 .- .Y3GC-!AG ti%J
- e a r l y c l i n i c a l
s u s p i c i o n l e a d i n g t o p r o d u c t i o n o f
v a c c i n e .
- type
o f v a c c i n e :
o r d i n a r y
c u l t u r e
m e d i u m o f P .
m u l t o c i d a t y p e E f c u l t u r e o n gelose w i t h blood + f o r m o l f
potash alum.
- v a c c i n a t i o n t w i c e a y e a r .
-8. Diaznosis
- c l i n i c a l d i a g n o s i s net. very e a s y .
- e x p e r i m e n t a l d i a g n o s i s b y i n o c u l a t i o n a n d c u l t u r e .
ex: 14
h e a r t h - b r e a k s ; 6 6
i n f e c t i o n s
and
39
deaths.
vacc i nat i on
301.33 i n c a t t l e a n d 4 1 7 i n s h e e p .
PASTEURELLOSIS WITH THE STERNE’S APPARATUS
A. A-!ne-I.j.qrat; jon of yacc: ! ne prcq1i.z t iqj
-
i m p o r t a n t . oer-7od
!Jps:
acauisition
cf 2
sterne’s
apparatus :
“fermer~top wl thout. Lt-:ermal
regul at.iir nut.
1 n to
a t-i
I ncubator chamber”.
- production medium 1~1th pancreas digest.ed in napain
(1n p l a c e o f the sterne’s panc.reas mediumi.
- c o n s t a n t
v e n t. i 1 a t i 0 3
an0
!‘E: g 1-J 1 .Tj !
s u p p 1 y’
o f
new
mec-1 i um .
8 . .-E-t i CJ ! og-x-7 ..s t ud I es
- t h e e n t i re country : s covered by the disease w:th
emphas: s on the south zone,
- g e r m
identified:
P.
Ii?U 1 toc ,d3,
9.
haenx l?ft: ica,
P.
pneumotrop ica and Pasteure i ia sp.
- study o n a n t i g e n i c struct.ure
Sasis f o r v a c c i n e p r o d u c t i o n : !?tterature review
o n af7 t 1 Cjti?Il i i7 a n a l y s i s a n d geographycal distr~butlon.
v a c c i n e a g a i n s t :
. ho\\.! I ne haemor-rhag i c septi caemi a wi t.h P.
ni!) 1 toc ida tyne E .
smal?
r u m 1 ri an t,
oasteur-e ! !OS’! 6
Witzl?
P.
multocida tyDe P a n d D.
. .2’/li;,l
4.
cho!er-a wi Zh P . mu ltoc 7da t.vDe A.
~3 f7 1:. ‘1
g e r? ‘1
c
ana1\\/s15
were done
i n
1 FMVT -f ‘France ‘)
i aDi)t-at,or-v ,
C.,._,gp ide!Ajmjgg..j..cgj __.. s.t-qbjcfes
tr _ -s.!Lg-y!%
s t a r t i n g p o i n t :
germ known leads to systemati c
v a c c i n a t i o n .
o b j e c t i v e s :
Perfect
the
e p i d e m i o l o g i c a l
knowledge in three areas:
g t i 0 1 ogicg]
.__-_-.-
..__ c,.ot.np-1 e-x
Pchw-~. rratb9e.v i c PaWc-el 1 a
b o v i n e h a e m o r r h a g i c s e p t i c a e m i a tybe E
i n S e n e g a l a n d primat-ily i n t h e s o u t h .
a v i a n
choiera
type A
f r e q u e n t i n
Senegal .
se.~o7bary_p_-~hog.enj.c Pa~teu.ce.1 1 a
i n P e s t e d e s P e t i t s R u m i n a n t s ( P P R 1:
primary
v i r a l
i n f e c t i o n
w i t h
secondary
i n v o l v e m e n t o f P.
m u l t o c i d a t y p e A 3 o r P . h a e m o lyt i c a and P a s t e u r e 7 la sp.
NB:
respi ratory
symtomps
leading
usual l y i n
m i s-Lake o r
confusion between PPR and Pasteurel losis.
P a s t e u r e l fosis i n v o l v e m e n t 1s k2ossi b l e aut
n o t abs.01 ute.
i- n-- .._.. Pneqmo~-op.a~.h~-es
-. .-..-.
:
m u l t i f a c t o r i a l
e t i o l o g y .
germ found are: P, multocida a n d P . haetno lvtica.
a s s o c i a t i o n k.nown: P .
mu 1 toc ida + Mycop lasma ov ipr?eum:?n
iae i n
pneumony’s lesions;
o
r
P
.
haemo7ytica -type 9
i n
s heep ’ s
cerebral t i s s u e s t r u c k d o w n b y oestrosjs ( O e s t r u s ovis’!.
s _ t u d y
._._.- -.__ o n
_ _ -...c o
_ n v e y .a r! c e o i:. p0.t; t. .g r. ag e
i~~.--sh-e.eP
samg 1 i big
î+-,ud
_ _
i ed :
lung,
t r a c h e a ,
1 av-ynx , cornet. ar?d front.al sinus 1 ining rnucosa.
ge r-m
isolated:
P.
ml./ 7 toc ida t..ype .41 ,
‘1 .:a\\., > A 7 f P 3 , A9 and DL.
<..,c- . hear!io Ï L t 7 C?l tyoe 1 ,
;
:g
a?:? 9
.
:j i .>s t, r 1 b ~1 t, i o r! frorn s i n u s l3 ~a~yr7~
:n a
clecrec,se trefid ( tract-tea aiid !~.-rrlg ger-m free) .
e v I ! 1 i-1 t. 7 0 n :
h cl a 1 t h i/ ;y p 1 ma 1
,;- 3. .‘. *- , e b- ‘\\A,) y+y k-,
bad ~~r!ysÏoiogical
state i e a d s t.o s i cL.ness,
!.t~‘...903t.s
same methodo 1 ogy 3s +‘(->r. sheer .
N B :
P .
h e a m o lyt ic:a III~“.~ -f p-.equent. 1 Iq
goay. rk,ar! i n sheep; and P,
*nu 7 toc ! da t y2e 42 mer-e fk-equenf::. i y!
. 1-
g9a .C
sa. ‘1 d
sheep ;
c0nveyav~c.e
g rester
i n
u p p e r
i i r 1.1 s
l e v e l than
i n
1 I, r y n Y
level ;
and
r~egu 1 ar
assoc i :at. 7 on
w i t )i
M.Ycor2 Iasma
HF g ir? if7 7
i !7 goats
a n d sheep,
j-n.. caii.t.- 1 g
same methodol ogy a s above
f reouerit
i n f e c t i o n
f rom
S 1 alJg’thet’-
house sampl i t7g : si nus and ohar-nx ,
ge t-m
1 SO 1 at.ed :
P.
f?)Ll : t2c 7 da
,t. y p e
A 1
ano Ai,
P.
h3emo 7.vt i (3 tuoe 6 .
Ii and iS.
asscc i at 1 on :
P.
Iî<~t>r?ip : ir’t’ 7 <:a
A
r4y1;c>p1 i;gri.~
0 (2 :,i : s
( causa;
g e r m o f b o v i :ie F~~:O~~Y : ::, :!neumovii ae i i ‘7
t !-t e u u ne r- t-espi ratorv t rack .
play a role i n human i n f e c t i o n b y b i t e
oriand scrat.chs.
b u c c a l
conveyance
for:
P.
mu 1 toc ida
t y p e A3, A 7 a n d A9.
i.n --WL LRa.t.t.v-3.. ra.cus,
r a t s i n 1 a b o r a t o r y c o m p o u n t .
b u c c a l c o n v e y a n c e f o r P . p n e u m o t r o p i c a
a n d P . multncida.
,
import.ant e p i d e m i o l o g i c a l fact.
se
-... f-0 1
_---ogi..cal.-~-~r_e~s
.j.J> .._ smal 1
rumi-_nt--p.~-~.~monopathles
.
b a s i s :
i ts
m u l t i f a c t o r i a l
a n d
s e q u e n t i a l f o r - m .
use
o f
sui ted
method
f o r
r u r a l
envi ronment :
p r o s p e c t i v e
t y p e o f
1 engthwi se
i l o n g i t u d i n a l )
s e r o - e p i d e m i o l o g i c a l s t u d y .
. kinetic s t u d y o f a n t i b o d i e s dur~ng
i nf ect i ous
e v e n t s : b e s t dignostical
v a l u e .
. s e r a w e r e t.ested for v i t-a1 , ch1 a m y s
ai;d myc«(jlasmic i n f e c t i o n s a n d a l s o f o r P .
multtxida
(P apd D‘I
a n d :3 serotypes o f F . haemolytica.
s e r o l o g i c a l
met.hod u s e d w a s : H . A . P .
( p a s s i v e haemagglutination).
. d a t a a n a l ysis bas s t a r t e d .
i Q. .~i~ag.n.s_t-.Ic_..tre__e.~,~.
.r~~e.t-todo 1 ogy
P a s t e u r e l l o s i s
and
P P R
i n d i r e c t
study b
y
vacc i nat i on
agai n s t
p a s t e u r e l losis i n
September
( r a i n y s e a s o n 1 a n d i n M a r s (cold d r y s e a s o n ) a n d a g a i n s t P P R
o n c e a year.
var i abl es
resu 1 ts
b y
‘: i t.e
ar?d
species.
PASTEURELLOSIS WITH MODERN BIOFERMENTORS
.i?ctua? s t a t e : n u m e r o u s r e q u e s t s .
bl e i-1
0 0 D 0 r t u n i t 1 e s :
p r o(i u c. t s
ti i 0 h 1 y .‘: ~!VI 0 e t. e r 1 ‘4 e
1 17
0 j_: *. 1 .I t v
ayld quant-i t.y .
SUMMARY
REPUBLIQUE DU SENEGAL
-----
MINISTERE DU DEVELOPPEMENT
RURAL ET DE L'HYDRAULIQUE
-----
INSTITUT SENEGALAIS DE RJXHERCHES
AGRICOLES (1-S-R-A.)
-w--m
DIRECTION DES RECHERCHES SUR LES
5-Y
PRODUCTIONS ET LA SANTJZ ANIMALES
y!/,
--e-w
G
LABORATOIRE NATIONAL DE L'ELEVAGE
ET DE RECHl$RCHES VETERINAIRES
DAKAR/HANN
ANIMAL PASTEURELLOSIS
The Senegalese experience
Dr. Mamady KONTE
Paper presented at the "International
REF, No 12 /PATHo. ANIM.
Workshop en Pasteurellosis in Animal
AVRIL 1992
Production", Bali, Indonesia,
loth - 13th 1992.
/
ANIMAL PASTEURELLOSIS :
THE SENEGALESE EXPERIENCE.
INTRODUCTION
- m a r k e d interest i n pasteurellcsis
since 1930.
- p r o d u c t i o n o f p o u l t r y ,
cat.t!e and sheep vaccines despi te
the u s e o f unsuited b u i l d i n g .
- i n t e n s i f i c a t i o n o f
diagnosis
activit.ies
and
vacc’i ne
p r o d u c t i o n aft.er m o v i n g i n s u i t e d b u i l d i n g .
organization
and
i n t e n s i f i c a t i o n o f
r e s e a r c h o n
epidemiology.
- b r i g h t e r f u t u r e
a n d prospects f o r r e s e a r c h o n a n i m a l
pathologies
a f t e r a c q u i s i t i o n o f m o d e r n f e r m e n t o r .
PASTEURELLOSIS BEFORE THE USE OF FERMENTOR
A2 .- .Y3GC-!AG ti%J
- e a r l y c l i n i c a l
s u s p i c i o n l e a d i n g t o p r o d u c t i o n o f
v a c c i n e .
- type
o f v a c c i n e :
o r d i n a r y
c u l t u r e
m e d i u m o f P .
m u l t o c i d a t y p e E f c u l t u r e o n gelose w i t h blood + f o r m o l f
potash alum.
- v a c c i n a t i o n t w i c e a y e a r .
-8. Diaznosis
- c l i n i c a l d i a g n o s i s net. very e a s y .
- e x p e r i m e n t a l d i a g n o s i s b y i n o c u l a t i o n a n d c u l t u r e .
ex: 14
h e a r t h - b r e a k s ; 6 6
i n f e c t i o n s
and
39
deaths.
vacc i nat i on
301.33 i n c a t t l e a n d 4 1 7 i n s h e e p .
PASTEURELLOSIS WITH THE STERNE’S APPARATUS
A. A-!ne-I.j.qrat; jon of yacc: ! ne prcq1i.z t iqj
-
i m p o r t a n t . oer-7od
!Jps:
acauisition
cf 2
sterne’s
apparatus :
“fermer~top wl thout. Lt-:ermal
regul at.iir nut.
1 n to
a t-i
I ncubator chamber”.
- production medium 1~1th pancreas digest.ed in napain
(1n p l a c e o f the sterne’s panc.reas mediumi.
- c o n s t a n t
v e n t. i 1 a t i 0 3
an0
!‘E: g 1-J 1 .Tj !
s u p p 1 y’
o f
new
mec-1 i um .
8 . .-E-t i CJ ! og-x-7 ..s t ud I es
- t h e e n t i re country : s covered by the disease w:th
emphas: s on the south zone,
- g e r m
identified:
P.
Ii?U 1 toc ,d3,
9.
haenx l?ft: ica,
P.
pneumotrop ica and Pasteure i ia sp.
- study o n a n t i g e n i c struct.ure
Sasis f o r v a c c i n e p r o d u c t i o n : !?tterature review
o n af7 t 1 Cjti?Il i i7 a n a l y s i s a n d geographycal distr~butlon.
v a c c i n e a g a i n s t :
. ho\\.! I ne haemor-rhag i c septi caemi a wi t.h P.
ni!) 1 toc ida tyne E .
smal?
r u m 1 ri an t,
oasteur-e ! !OS’! 6
Witzl?
P.
multocida tyDe P a n d D.
. .2’/li;,l
4.
cho!er-a wi Zh P . mu ltoc 7da t.vDe A.
~3 f7 1:. ‘1
g e r? ‘1
c
ana1\\/s15
were done
i n
1 FMVT -f ‘France ‘)
i aDi)t-at,or-v ,
C.,._,gp ide!Ajmjgg..j..cgj __.. s.t-qbjcfes
tr _ -s.!Lg-y!%
s t a r t i n g p o i n t :
germ known leads to systemati c
v a c c i n a t i o n .
o b j e c t i v e s :
Perfect
the
e p i d e m i o l o g i c a l
knowledge in three areas:
g t i 0 1 ogicg]
.__-_-.-
..__ c,.ot.np-1 e-x
Pchw-~. rratb9e.v i c PaWc-el 1 a
b o v i n e h a e m o r r h a g i c s e p t i c a e m i a tybe E
i n S e n e g a l a n d primat-ily i n t h e s o u t h .
a v i a n
choiera
type A
f r e q u e n t i n
Senegal .
se.~o7bary_p_-~hog.enj.c Pa~teu.ce.1 1 a
i n P e s t e d e s P e t i t s R u m i n a n t s ( P P R 1:
primary
v i r a l
i n f e c t i o n
w i t h
secondary
i n v o l v e m e n t o f P.
m u l t o c i d a t y p e A 3 o r P . h a e m o lyt i c a and P a s t e u r e 7 la sp.
NB:
respi ratory
symtomps
leading
usual l y i n
m i s-Lake o r
confusion between PPR and Pasteurel losis.
P a s t e u r e l fosis i n v o l v e m e n t 1s k2ossi b l e aut
n o t abs.01 ute.
i- n-- .._.. Pneqmo~-op.a~.h~-es
-. .-..-.
:
m u l t i f a c t o r i a l
e t i o l o g y .
germ found are: P, multocida a n d P . haetno lvtica.
a s s o c i a t i o n k.nown: P .
mu 1 toc ida + Mycop lasma ov ipr?eum:?n
iae i n
pneumony’s lesions;
o
r
P
.
haemo7ytica -type 9
i n
s heep ’ s
cerebral t i s s u e s t r u c k d o w n b y oestrosjs ( O e s t r u s ovis’!.
s _ t u d y
._._.- -.__ o n
_ _ -...c o
_ n v e y .a r! c e o i:. p0.t; t. .g r. ag e
i~~.--sh-e.eP
samg 1 i big
î+-,ud
_ _
i ed :
lung,
t r a c h e a ,
1 av-ynx , cornet. ar?d front.al sinus 1 ining rnucosa.
ge r-m
isolated:
P.
ml./ 7 toc ida t..ype .41 ,
‘1 .:a\\., > A 7 f P 3 , A9 and DL.
<..,c- . hear!io Ï L t 7 C?l tyoe 1 ,
;
:g
a?:? 9
.
:j i .>s t, r 1 b ~1 t, i o r! frorn s i n u s l3 ~a~yr7~
:n a
clecrec,se trefid ( tract-tea aiid !~.-rrlg ger-m free) .
e v I ! 1 i-1 t. 7 0 n :
h cl a 1 t h i/ ;y p 1 ma 1
,;- 3. .‘. *- , e b- ‘\\A,) y+y k-,
bad ~~r!ysÏoiogical
state i e a d s t.o s i cL.ness,
!.t~‘...903t.s
same methodo 1 ogy 3s +‘(->r. sheer .
N B :
P .
h e a m o lyt ic:a III~“.~ -f p-.equent. 1 Iq
goay. rk,ar! i n sheep; and P,
*nu 7 toc ! da t y2e 42 mer-e fk-equenf::. i y!
. 1-
g9a .C
sa. ‘1 d
sheep ;
c0nveyav~c.e
g rester
i n
u p p e r
i i r 1.1 s
l e v e l than
i n
1 I, r y n Y
level ;
and
r~egu 1 ar
assoc i :at. 7 on
w i t )i
M.Ycor2 Iasma
HF g ir? if7 7
i !7 goats
a n d sheep,
j-n.. caii.t.- 1 g
same methodol ogy a s above
f reouerit
i n f e c t i o n
f rom
S 1 alJg’thet’-
house sampl i t7g : si nus and ohar-nx ,
ge t-m
1 SO 1 at.ed :
P.
f?)Ll : t2c 7 da
,t. y p e
A 1
ano Ai,
P.
h3emo 7.vt i (3 tuoe 6 .
Ii and iS.
asscc i at 1 on :
P.
Iî<~t>r?ip : ir’t’ 7 <:a
A
r4y1;c>p1 i;gri.~
0 (2 :,i : s
( causa;
g e r m o f b o v i :ie F~~:O~~Y : ::, :!neumovii ae i i ‘7
t !-t e u u ne r- t-espi ratorv t rack .
play a role i n human i n f e c t i o n b y b i t e
oriand scrat.chs.
b u c c a l
conveyance
for:
P.
mu 1 toc ida
t y p e A3, A 7 a n d A9.
i.n --WL LRa.t.t.v-3.. ra.cus,
r a t s i n 1 a b o r a t o r y c o m p o u n t .
b u c c a l c o n v e y a n c e f o r P . p n e u m o t r o p i c a
a n d P . multncida.
,
import.ant e p i d e m i o l o g i c a l fact.
se
-... f-0 1
_---ogi..cal.-~-~r_e~s
.j.J> .._ smal 1
rumi-_nt--p.~-~.~monopathles
.
b a s i s :
i ts
m u l t i f a c t o r i a l
a n d
s e q u e n t i a l f o r - m .
use
o f
sui ted
method
f o r
r u r a l
envi ronment :
p r o s p e c t i v e
t y p e o f
1 engthwi se
i l o n g i t u d i n a l )
s e r o - e p i d e m i o l o g i c a l s t u d y .
. kinetic s t u d y o f a n t i b o d i e s dur~ng
i nf ect i ous
e v e n t s : b e s t dignostical
v a l u e .
. s e r a w e r e t.ested for v i t-a1 , ch1 a m y s
ai;d myc«(jlasmic i n f e c t i o n s a n d a l s o f o r P .
multtxida
(P apd D‘I
a n d :3 serotypes o f F . haemolytica.
s e r o l o g i c a l
met.hod u s e d w a s : H . A . P .
( p a s s i v e haemagglutination).
. d a t a a n a l ysis bas s t a r t e d .
i Q. .~i~ag.n.s_t-.Ic_..tre__e.~,~.
.r~~e.t-todo 1 ogy
P a s t e u r e l l o s i s
and
P P R
i n d i r e c t
study b
y
vacc i nat i on
agai n s t
p a s t e u r e l losis i n
September
( r a i n y s e a s o n 1 a n d i n M a r s (cold d r y s e a s o n ) a n d a g a i n s t P P R
o n c e a year.
var i abl es
resu 1 ts
b y
‘: i t.e
ar?d
species.
PASTEURELLOSIS WITH MODERN BIOFERMENTORS
.i?ctua? s t a t e : n u m e r o u s r e q u e s t s .
bl e i-1
0 0 D 0 r t u n i t 1 e s :
p r o(i u c. t s
ti i 0 h 1 y .‘: ~!VI 0 e t. e r 1 ‘4 e
1 17
0 j_: *. 1 .I t v
ayld quant-i t.y .
SUMMARY