NuMERA'i'ION DES (Z&?.ES VIVANTS TABLE DE...
NuMERA'i'ION DES (Z&?.ES VIVANTS
TABLE DE TAYLOR (1962)
Humération des germes vivants
Table de Taylor (7962)
Extrait de "Théory and practice in expedmental &cteriolo&'
1965,
p. 204
Tables 6.10 - 6.12, '3'he most p-robable number (Y.P.N.1 of organisms in
viable counts by the dilution mthod
Each of these tables has been computed for a dilution factor of 10, and for
either 5, 8, or 10 tubes inoculated frzm each dilution of culture (Tables 6.10,
6.11, 6.12 respec-tively).
The en-tries in each table are the numbers of turbid tubes observed after
inoculation of the same volume fr~m any three successive lO-fold dilutions. ?'he
statcd V.P.M. is the estin-ated mzan viable Count pc.r inoculum taken from the rxxt
concentrated suspension.
banple 1 - Suppse 1 CL was inoculated. tim the 10°, 10-' and 10-'dilutions
of culture into 5 tubes for each dilution, and that the n~nxikrs of turbid tubes
observed after incubation were 4-2-l. Table 6.10 shcxs that the M.P.Y. = 2.6 per
inoculumtaken from the 10' dilution.
ExampleZ - Supxxe the same rxsults xer+e obtained with tubes inoculated
from 10s3, 10-" and lO-' dilutions. The 'LT.? .N. is thcn 2.6 psr inoculum taken froc
the 10-2 dilution, or 2.6 x 103 pur inoculum of undiluted culture.
The tables include only those combinationp 3 of results likely to be compati-
ble with equation 6.3 : the probability that a given result would be obtained
from the stated M.P.?i. is provided in the original papers.
The published form of these tables diffws sliphtly frorn that used here as
they were oriiginally computed for a different for-m of th? dilution count. Instead
of making serial dilutions of culture and takinp= the same volume .from each dilu-
tion for inoculation, as described here, the culte was no-t diluted but different
volumes of inoculum werc?l taken froc, it, for exar@e 10, 1, and 0.1 ml, the resul-
ting differences in thc concrx-kration of culture medium beine imored. The pu-
blished tables therefoxx give the LV.P.N./CL. or per 100 ml cüL-tu~.
. . ./ . . .
-2-
Table 6.10 - Values of tlr,c Yi.P.N. for 5 tubzs -inoculat& fyr?m cach of
thrxe successive 10 - f0l.d dilutions (Taylor, 1962)
-..e-
.I
-a---..,-
--..--.-. -
VI. __-_ - _ILf -.-~--.
.s
-...-..-
..-
1
l+-nvbers of
hlulnb~m of
I
tu&id tubes
twbid tubes
observed a-t thEe
obscmm! at thEe
f
successive
M.P.N.
succcssi-w
M.P.M.
1
dilutions
(per inoculw of thi!
dilutions
(ner inoculm of :
first dilution)
khe first dilutic@
I
t
1
‘0
-
1
0’
O.J.8
5
2.3
1I
1
0
0
0.30
5
3.3. ;
1
1
0
C-I.40
5
3.3
I
2
0
0
0.45
5
4.6
!
2
0
1
0.68
5
4.9
j
2
1
--
0
0.68
5
7.0
i
2
!
2
0
10.93
5
9.s
1
..*
3
0
0
0.78
5
7.9
3
0
1
l . 1
5\\.
11.0
/
!
3
1
0
1.1
5
14.0
/
I
3
2
0
l.4
5
13.0
l
4
0
0
1.3
,s
17.3
l
4
0
1
1.7
S
22.0
i
4
1
0
1.7
5
\\
28.0
i
1
4
1
1
2.1
5
24.1)
I
1
t
4
2
0
2.2
c\\.
35.0
i
t
4
2
1
2.6
5
54.0
[
I
4
3
9
2 . 7
I
s
92 CO
!
i
5
160.0
:
--~ru..a~-~--r--~-..
--
.-s --LI
.^___
TABLE DE TAYLOR (1962)
Humération des germes vivants
Table de Taylor (7962)
Extrait de "Théory and practice in expedmental &cteriolo&'
1965,
p. 204
Tables 6.10 - 6.12, '3'he most p-robable number (Y.P.N.1 of organisms in
viable counts by the dilution mthod
Each of these tables has been computed for a dilution factor of 10, and for
either 5, 8, or 10 tubes inoculated frzm each dilution of culture (Tables 6.10,
6.11, 6.12 respec-tively).
The en-tries in each table are the numbers of turbid tubes observed after
inoculation of the same volume fr~m any three successive lO-fold dilutions. ?'he
statcd V.P.M. is the estin-ated mzan viable Count pc.r inoculum taken from the rxxt
concentrated suspension.
banple 1 - Suppse 1 CL was inoculated. tim the 10°, 10-' and 10-'dilutions
of culture into 5 tubes for each dilution, and that the n~nxikrs of turbid tubes
observed after incubation were 4-2-l. Table 6.10 shcxs that the M.P.Y. = 2.6 per
inoculumtaken from the 10' dilution.
ExampleZ - Supxxe the same rxsults xer+e obtained with tubes inoculated
from 10s3, 10-" and lO-' dilutions. The 'LT.? .N. is thcn 2.6 psr inoculum taken froc
the 10-2 dilution, or 2.6 x 103 pur inoculum of undiluted culture.
The tables include only those combinationp 3 of results likely to be compati-
ble with equation 6.3 : the probability that a given result would be obtained
from the stated M.P.?i. is provided in the original papers.
The published form of these tables diffws sliphtly frorn that used here as
they were oriiginally computed for a different for-m of th? dilution count. Instead
of making serial dilutions of culture and takinp= the same volume .from each dilu-
tion for inoculation, as described here, the culte was no-t diluted but different
volumes of inoculum werc?l taken froc, it, for exar@e 10, 1, and 0.1 ml, the resul-
ting differences in thc concrx-kration of culture medium beine imored. The pu-
blished tables therefoxx give the LV.P.N./CL. or per 100 ml cüL-tu~.
. . ./ . . .
-2-
Table 6.10 - Values of tlr,c Yi.P.N. for 5 tubzs -inoculat& fyr?m cach of
thrxe successive 10 - f0l.d dilutions (Taylor, 1962)
-..e-
.I
-a---..,-
--..--.-. -
VI. __-_ - _ILf -.-~--.
.s
-...-..-
..-
1
l+-nvbers of
hlulnb~m of
I
tu&id tubes
twbid tubes
observed a-t thEe
obscmm! at thEe
f
successive
M.P.N.
succcssi-w
M.P.M.
1
dilutions
(per inoculw of thi!
dilutions
(ner inoculm of :
first dilution)
khe first dilutic@
I
t
1
‘0
-
1
0’
O.J.8
5
2.3
1I
1
0
0
0.30
5
3.3. ;
1
1
0
C-I.40
5
3.3
I
2
0
0
0.45
5
4.6
!
2
0
1
0.68
5
4.9
j
2
1
--
0
0.68
5
7.0
i
2
!
2
0
10.93
5
9.s
1
..*
3
0
0
0.78
5
7.9
3
0
1
l . 1
5\\.
11.0
/
!
3
1
0
1.1
5
14.0
/
I
3
2
0
l.4
5
13.0
l
4
0
0
1.3
,s
17.3
l
4
0
1
1.7
S
22.0
i
4
1
0
1.7
5
\\
28.0
i
1
4
1
1
2.1
5
24.1)
I
1
t
4
2
0
2.2
c\\.
35.0
i
t
4
2
1
2.6
5
54.0
[
I
4
3
9
2 . 7
I
s
92 CO
!
i
5
160.0
:
--~ru..a~-~--r--~-..
--
.-s --LI
.^___