l ? :. .3 INSTITUT SENEGALAIS DE RECHERCHES...
l ? :. .3
INSTITUT SENEGALAIS DE RECHERCHES AGRICOLES
’CT &--:
DIRECTION DES RECHERCHES SUR LES PRODUCTIONS FORESTIERES
CENTRE DE RESSOURCES MICROBIOLOGIQUES (MIRCEN)
SOIL AMELIORATION WITH NITROGEN-FIXING
ACACIA SPECIES
F i n a l r e p o r t o f I A E A contract no.6375/Rl/RB
bY
Mamadou GUEYE, Mahamadl DIANDA, Ibrahima NDOYE and Bernard DREYFUS
INTRODUCTION
Previous study showed that Faidherbia albida has a low nitrogen
fixation potential (NFP). Therefore, it is necessary to enhance its N,
fixation in order to increase the ability of this species to improve soi1
fertility and restore the N status. One approach to reaching this goal is
to Select F. albida provenances with the best NFP.
This study aims to compare the nitrogen fixing potential of
different provenances of F. albida originated from Senegal and Burkina
Faso.
MATERIALS AND METHODS
Greenhouse experiment was carried out at Dakar (Bel-Air
experimental station) using local sandy soil (93% of soil). This soil has
a pH of 7.0, 1.9% total C and 0.25% total N. The soil was sieved (< 1
mm), homogeneized and placed into pots (20 Kg/pot) 30 cm in diameter.
TO each pot 1 g K,HPO, was added. Surface-sterilized seeds of Parkia
biglobosa and Faidherbia albida were germinated in petri dishes
containing soft agar 0.8% for 60 mn and 30 mn respectively. At 2 days
old, they were transplanted into the pots (1 seedling/pot).
There were eight treatments with five replicates : one non fixing
tree, i. e. Parkia biglobosa as reference crops and seven provenances of
Faidherbia albida from Senegal (Merina, Dangalma, Ndiongolor, Pire and
Kabrousse) and burkina Faso (Gomblora and Dem) inoculated with
Bradyrhizobium ORS 136 applied as liquid inoculum (10 ml/pot)
containing 10’ cells/ml. T O all p o t s , nitrogen fertilizer was then
applied at the rate of 0.2 g N/pot as a solution of (‘5N-l,),s0, with
10.01% atom excess. The pots were arranged randomly and watered SO
that the soil moisture was kept close to the field capacity.
At 6 months after transplantation into the pots, plants were
harvested. Plant and nodule dry weights were recorded. Nitrogen
content (%N) and %15N atom excess were determined by the IAEA
laboratory at Seibersdorf. Nitrogen fixation was estimated using both
isotope dilution and A-value methods.
5/01/94 - 2
Fin. Rpt
AIEA/6375/Rl/RB
RESULTS
P l a n t g r o w t h a n d nitrogen y i e l d
There were significant differences between F. albida provenances
in terms of %15N atom excess, dry matter (DM) and total nitrogen yield
(Table 1). For each plant part, the %15N atom excess of provenances of
Merina and Dangalma was higher than that of other trees including the
reference tree whereas the provenance of Kabrousse had the lowest
one. However, the provenance of Kabrousse seemed to yield more than
other provenances in terms of dry weight and total nitrogen of the
leaves and the stems even there was no significant difference between
the provenances in the roots and the whole plant in term of dry weight
and total nitrogen.
Nitrogen d e r i v e d f r o m f i x a t i o n
Whatever the method used, the proportion (%Ndfa) and amount
(Ndfa) of nitrogen derived from fixation are more important in the
provenance of Kabrousse than in the other provenances (Tables 2, 3, 4
and 5).
Refering to the isotope dilution technique (Tables 2 and 3), the
coefficient of variation was very high due to the variability between
the provenances : some of them (Merina and Dangalma) presented
negative values means that they did not fix nitrogen whereas the other
provenances showed a significant N, fixation even this fixation was
quite low, which was demonstrated in the previous study : the
pourcentage of nitrogen derived from fixation was -17.88%, -4.26%,
25.97%, 25.48%, 38.17%, 13.08 and 19.19% corresponding to an amount
of -0.06, -0.03, 0.26, 0.24, 0.44, 0.10 and 0.16 g N plant-’ for merina,
Dangalma, Ndiongolor, Pire, Kabrousse, Gomblora and Dem respectively.
Refering to A-value method (Tables 4 and 5), no negative value
was recorded. Nevertheless the provenances of Merina and Dangalma
fixed less nitrogen than the other provenances. The proportion of
nitrogen derived from fixation was 17.90%, 28.93%, 49,43%, 49.08%,
57.79%, 40.62% and 44.87% corresponding to an amount of 0.15, 0.21,
0.51, 0.44, 0.63, 0.31 and 0.36 g N plant-’ for Merina, Dangalma,
Ndiongolor, Pire, Kabrousse, Gomblora and Dem respectively. Similarly
to the isotope dilution technique, the coefficient of variation was high
due to the variability between the provenances.
5/01/94 - 3
Fin. Rpt
AIEA/6375/Rl/RB
Table 1. %15N atom excess, dry weight, nitrogen content and total nitrogen in different
plants parts of Parkia biglobosa and seven Faidherbia albida provenances cultivated in pots
containing 20 kg of non sterile soil.
____----------------------------.------------------------
Plant
T r e e
Dry weight
Total N
parts
species
Provenances*
%15NEX3
(g plant’)
%N
(g plant’)
- - - - - - - -
- - - - - -
-m-----m-
----
Leaves
P. biglobosa
0.691ab
1 3.35a
2.71b
F.albida
Merina (S)
0.776'
7.36'
2.87b
Dangalma (S)
0.745a
8.40bC
3.06b
Ndiongolor (S)
0.50gCd
12.18ab
2.75b
Pire (S)
0.523Cd
9.95ak
3.08b
Kabrousse (S)
0.415d
12.36ab
3.11b
Gomblora (BF)
0.596bC
6.66c
3.5ga
Dem (BF)
0.528Cd
7.80'
3.58*
c v (%)
14.60
24.10
8.70
24.50
Stems
P. biglobosa
0.64gbC
11.95bC
1.02c
0.12b
F. albida
Merina (S)
0.854'
7.77"
1.12bC
O.Ogb
Dangalma (S)
0.726"
9.10bC
1.18ak
O.llb
Ndiongolor (S)
0.49gCd
13.1 2abc
1.06bC
0.14ab
Pire (S)
0.496Cd
1 4.50ab
1.13bC
0.16ab
Kabrousse (S)
0.393d
18.43’
l.07bc
0.20a
Gomblora (BF)
0.581bcd
7.54c
1.37"
O.lOb
Dem (BF)
0.544bcd
9.80bC
1.28ab
0.13b
c v (%)
18.00
30.00
11.20
31.10
Roots
P. biglobosa
0.624ab
27.35"
1.28'
o.35a
F.albida
Merina (S)
0.729"
20.47"
1.73b
0.36a
Dangalma (S)
0.611ab
20.95a
1.76ab
0.37
Ndiongolor (S)
0.447bC
27.60a
1.8Pb
0.52"
Pire (S)
0.446bC
24.65"
1.66b
0.40a
Kabrousse (S)
o.404c
27.91a
1.84ab
0.50*
Gomblora (BF)
0.530bc
20.35a
2.06'
0.40a
Dem (BF)
0.511bC
19.51a
1.90ab
0.36"
c v (%)
19.70
34.10
9.60
34.80
Total
P. biglobosa
0.650abc
52.65a
1.67"
0.83a
F. albida
Merina (S)
0.780'
35.60a
1.91b
0-67
Dangalma (S)
0.700a
38.45a
2.00b
0.73a
Ndiongolor (S)
0.490de
52.90*
1 .90b
0.99*
Pire (S)
0.490d"
49.94"
1 .96b
0.87
Kabrousse (S)
0.410e
58.70"
2.01b
1.08*
Gomblora (BF)
0.570bcd
34.54*
2.34"
0.74"
Dem (BF)
o.530cde
37.11a
2.26'
0.77a
cv (%)
15.20
28.70
6.60
28.40
_____--_-------------------------------------------------
* : (BF) : Burkina Faso ; (S) : Senegal
For each plant part, values in the same column followed by the same letter do not differ
significantly at p = 0.05.
S/Ql/94-4
Fin. Rpt
AIEA/6375/Rl/RB
Table 2. Proportion of nitrogen derived from atmosphere (%Ndfa), fertilizer (%Ndff)
and soi1 (%Ndfs) in different plants parts of Parkia biglobosa and seven Faidherbia albida
provenances cultivated in pots containing 20 kg of non sterile soil using isotope dilution.
Plant
T r e e
parts
species
Provenances*
%Ndfa
%Ndff
%Ndfs
- - - - m----e- ----------_
- - - . - - -
- - - - - -
-m--m---
Leaves P. biglobosa
0.00
7.17ab
92.a3ab
F. albida
Merina (S)
-12.33"
8.05"
104.2aa
Dangalma (S)
- 7.87"
7.73"
100.14"
Ndiongolor (S)
26.34ab
5.2aCd
6a.3aCd
Pire (S)
24.31ab
5.43Cd
70.26Cd
Kabrousse (S)
39.a6a
4.31d
55.a3d
Gomblora (BF)
13.75b
6.1abC
a0.07bC
Dem (BF)
23.33ab
5.50Cd
71.1aCd
c v (%)
84.50
14.60
14.00
Stems
P. biglobosa
0.00
6.73bC
97.27bC
F. albida
Merina (S)
-31.65"
8.86"
122.79"
Dangalma (S)
-1 1.a6bC
7.53ab
104.33ab
Ndiongolor (S)
23.17a
5.1Td
71.65Cd
Pire (S)
23.64'
5.14Cd
71.22Cd
Kabrousse (S)
39.54a
4.07d
56.3gd
Gomblora (BF)
1 0.45ab
6.03bcd
a3.52bcd
Dem (BF)
16.15a
5.65bcd
7a.21Cd
c v (%)
174.70
la.00
17.70
Roots
P. biglobosa
0.00
6.47ab
95.53ab
F. albida
Merina (S)
-1 6.66b
7.56a
109.2ga
Dangalma (S)
2.05ab
6.34ab
91.61ab
Ndiongolor (S)
28.408
4.63bC
66.97bC
Pire (S)
28.498
4.63bC
66.aabC
Kabrousse (S)
35.13a
4.20'
60.67
Gomblora (BF)
15.00a
5.50bC
79.50bC
Dem (BF)
la.lia
5.30bC
76.5gbC
c v (%)
111.30
19.70
19.00
Total
P. biglobosa
0.00
6.7gabc
93.21ab
F. albida
Merina (S)
-17.aaC
a.16a
112.12a
Dangalma (S)
-4.26bC
7.20ab
9a.6aab
Ndiongolor (S)
25.97a
5.03d
69.00Cd
Pire (S)
25.4aa
C~06~
69.45cd
Kabrousse (S)
38.17a
4.6gd
57.63d
Gomblora (BF)
13.07ab
5.90bcd
81.03bC
Dem (BF)
19.1ga
5.4aCd
69.81Cd
c v (%)
100.60
15.10
15.50
_________---__-------------------------------------------
* : (BF) : Burkina Faso ; (S) : Senegal
For each plant part, values in the same column followed by the same letter do not differ
significantly at p = 0.05.
5/01/94 - 5
Fin. Rpt
AIEA/6375/Rl/RB
Table 3. Proportion of nitrogen derived from atmosphere (Ndfa), fettilizer (Ndff) and
soi1 (Ndfs) in different plants parts of Parkia biglobosa and seven Faidherbia aibida
provenances cultivated in pots containing 20 kg of non sterile soi1 using isotope dilution.
Plant
T r e e
Ndfa
Ndff
Ndfs
parts
species
Provenances*
(cl PL’)
(cl PL’)
kl PL’)
---_ - - - - - - -
- - - - - - -
- - - - - -
- - - - - -
Leaves P. biglobosa
0.00
0.030*
0.33*
F.albida
Merina (S)
-o.03c
0.020*
0.23*
Dangalma (S)
-o.02c
0.020*
0.26*
Ndiongolor (S)
O.Ogb
0.020*
0.23*
Pire (S)
0.07b
0.020*
0.22*
Kabrousse (S)
0.16'
0.020*
0.21*
Gomblora (BF)
0.03bC
0.01 o*
0.19*
Dem (BF)
0.06b
0.020*
0.15*
c v (%)
86.30
28.20
28.80
Stems
P. biglobosa
0.00
0.008*
0.1 la
F. albida
Merina (S)
-0.02d
0.008*
0.10*
Dangalma (S)
-O.OICd
0.008*
0.1 la
Ndiongolor (S)
0.03bC
0.007*
O.lOB
Pire (S)
0.04b
0.008*
0.1 la
Kabrousse (S)
0.08'
0.008"
0.10*
Gomblora (BF)
O.OiCd
0.006'
0.09*
Dem (BF)
0.02bcd
0.007*
o.oga
c v (%)
130.60
32.40
31.30
Fioots
P. biglobosa
0.00
0.020a
0.32a
F.a/bida
Merina (S)
-o.05c
0.030*
0.38a
Dangalma (S)
0.01 bc
0.020*
0.34*
Ndiongolor (S)
0.16ab
0.020*
0.34*
Pire (S)
0.12abc
0.020*
0.26'
Kabrousse (S)
0.20*
0.020*
0.29*
Gomblora (BF)
O.OGabC
0.020a
O-32*
Dem (BF)
0.08abc
0.020*
0.27
c v (%)
110.70
34.60
34.80
Total
P. biglobosa
0.00
0.05*
o.77a
F. albida
Merina (S)
-0.06'
0.05*
0.72*
Dangalma (S)
-o.03c
0.05*
0.71*
Ndiongolor (S)
0.26ab
0.05"
0.67"
Pire (S)
0.24ab
0,04*
0.59*
Kabrousse (S)
0.44*
0.05*
0.66*
Gomblora (BF)
O.lObC
0.04*
0.59*
Dem (BF)
0.16bC
0,04*
0.52*
c v (%)
95.40
34.10
29.40
* : (BF) : Burkina Faso ; (S) : Senegal
For each plant part, values in the same column followed by the same letter do not differ
significantly at p = 0.05.
5/01/94 - 6
Fin. Rpt
AIEA/6375/Ri/RB
Table 4. Proportion of nitrogen derived from atmosphere (%Ndfa), fertilizer (%Ndff)
and soi1 (%Ndfs) in different plants parts of Parkia biglobosa and seven Faidherbia albida
provenances cultivated in pots containing 20 kg of non sterile soil using A value method.
_____---------------------------------------------------
Plant
T r e e
parts
species
Provenances*
%Ndfa
%Ndff
%Ndfs
-__- - - - - - - -
---------_-
- - - - - -
- - - - - -
Leaves P. biglobosa
7.17ab
92.83”
F.albida
Merina (S)
20.38"
8.05'
71 .57b
Dangalma (S)
23.54"
7.73"
68.73b
Ndiongolor (S)
47.7Pb
5.28Cd
46.93Cd
Pire (S)
46.35ab
5.43Cd
48.22Cd
Kabrousse (S)
57.37a
4.31d
38.32d
Gomblora (BF)
38.86b
6.18bC
54.96’
Dem (BF)
45.65ab
5.50Cd
48.85Cd
c v (%)
23.00
14.60
13.10
Stems
P. biglobosa
6.73bC
93.27
F.aibida
Merina (S)
9.91C
8.86"
81.22ab
Dangalma (S)
23.45bC
7.53ab
69.02b
Ndiongolor (S)
47.43a
5.17Cd
47.40c
Pire (S)
47.74a
5.14Cd
47.1 lC
Kabrousse (S)
58.53a
4.07d
37.40"
Gomblora (BF)
38.72ab
6.03bcd
55.25'
Dem (BF)
42.62"
5.65bcd
51.73O
c v (%)
31.00
18.00
16.06
Roots
P. biglobosa
6.47ab
93.53”
F. albida
Merina (S)
23.42b
7.56a
69.02b
Dangalma (S)
39.8 Ila
6.34ab
57.85bC
Ndiongolor (S)
53.08a
4.63"
42.2gcd
Pire (S)
53.14a
4.63bC
42.23Cd
Kabrousse (S)
57.4ga
4.20'
38.31d
Gomblora (BF)
44.3aa
5.50bC
50.20Cd
Dem (BF)
46.33"
5.30bC
48.3Td
c v (%)
26.80
19.70
17.60
Total
P. biglobosa
93.21a
F. albida
Merina (S)
1 7.90c
73.94b
Dangalma (S)
28.93k
65.20'
Ndiongolor (S)
49.43a
45.54Cd
Pire (S)
49.08’
45.86Cd
Kabrousse (S)
57.7ga
38.01d
Gomblora (BF)
40.62ab
53.47
Dem (BF)
44.87"
49 .65cd
c v (%)
23.10
15.10
13.70
---------------------------------------------------------
* : (BF) : Burkina Faso ; (S) : Senegal
For each plant part, values in the same column followed by the same letter do not differ
significantly at p = 0.05.
5/01/94 - 7
F i n . Rpt
AIEA16375/Ri/RB
Table 5. Proportion of nitrogen derived from atmosphere (Ndfa), fertilizer (Ndff) and
soil (Ndfs) in different plants parts of Parkia biglobosa and seven Faidherbia albida
provenances cultivated in pots containing 20 kg of non sterile soil using A value method.
Plant
T r e e
Ndfa
Ndff
Ndfs
parts
species
Provenances*
(g PU
(9 pl.-‘)
(g Pl.“)
- - - - - - - - - - -
-a---------
---.---_
- - - - - -
- - - - - -
Leaves P. biglobosa
0.030a
0.33a
F.albida
Merina (S)
o.osd
0.020a
0.16b
Dangalma (S)
0.06Cd
0.020a
O.lab
Ndiongolor (S)
0.16b
0.020"
0.15b
Pire (S)
O.wb
0.020"
0.15b
Kabrousse (S)
0.22a
o.020a
0.14b
Gomblora (BF)
O.OSbCd
O.O1oa
0.13b
Dem (BF)
0.13bC
o.020a
0.14b
c v (%)
36.20
28.20
28.6
Stems
P. biglobosa
O.OOaa
F.albida
Merina (S)
O.OOaa
Dangalma (S)
O.OOaa
Ndiongolor (S)
0.007a
Pire (S)
O.OOaa
Kabrousse (S)
0.008"
Gomblora (BF)
0.006"
Dem (BF)
0.0078
c v (%)
52.00
32.40
31.00
Roots
P. biglobosa
o.020a
F. albida
Merina (S)
0.030"
Dangalma (S)
0.020a
Ndiongolor (S)
o.020a
Pire (S)
o.020a
Kabrousse (S)
0.020"
Gomblora (BF)
0.020"
Dem (BF)
o.020a
c v (%)
50.20
34.60
33.20
Total
P. biglobosa
0.050a
F.albida
Merina (S)
0.15"
0.050a
Dangalma (S)
0.21C
0.050a
Ndiongolor (S)
0.51ab
o.050a
Pire (S)
o.44abc
0.040a
Kabrousse (S)
0.63a
o.050a
Gomblora (BF)
0.31bC
o.040a
Dem (BF)
0.36abc
0.040a
c v (%)
42.60
34.10
27.40
__________----_------------------------------------------
* : (BF) : Burkina Faso ; (S) : Senegal
For each plant part, values in the same column followed by the same letter do not differ
significantly at p = 0.05.
5101184 - 8
F i n . Rpt
AlEA16375/Rl/RB
Nitrogen derlved f r o m f e r t i l i z e r and soi/
The proportion of nitrogen uptaken from fertilizer by ail F. albida
provenances was very low. It ranged from 4.69% in the provenance of
kabrousse to 8.16% in that of Merina. The proportion of nitrogen derived
from soi1 (%Ndfs) was higher in the provenances of Merina and
Dangalma than in the other provenances species. However, there was no
significant difference between the provenances in term of amount of
nitrogen derived from fixation and soil.
CONCLUSION
F. albida from Merina and Dangalma presented a very low N,
fixation. The provenance of Kabrousse was that with the higher NFP. It
should be selected for improving the soi1 fertlity SO far F. albida is
considered as a nitrogen fixing tree with an important agronomie
characteristic.
However, it is necessary to estimate the N, fixation in field
condition of the provenance of Kabrousse. This is the aim of the
renewalled contract (contract no. 6375/R2/RB) we have initiated.
We also keep in mind the another approach which involve grafting
the provenances of Merina and Dangalma onto that of Kabrousse or
grafting the provenance of Kabrousse onto Acacia seyal. This is also
initiated in the second renewal of the contract.
5/01/94 - 9
Fin. Rpt
AIEA/6375/Rl/RB
INSTITUT SENEGALAIS DE RECHERCHES AGRICOLES
’CT &--:
DIRECTION DES RECHERCHES SUR LES PRODUCTIONS FORESTIERES
CENTRE DE RESSOURCES MICROBIOLOGIQUES (MIRCEN)
SOIL AMELIORATION WITH NITROGEN-FIXING
ACACIA SPECIES
F i n a l r e p o r t o f I A E A contract no.6375/Rl/RB
bY
Mamadou GUEYE, Mahamadl DIANDA, Ibrahima NDOYE and Bernard DREYFUS
INTRODUCTION
Previous study showed that Faidherbia albida has a low nitrogen
fixation potential (NFP). Therefore, it is necessary to enhance its N,
fixation in order to increase the ability of this species to improve soi1
fertility and restore the N status. One approach to reaching this goal is
to Select F. albida provenances with the best NFP.
This study aims to compare the nitrogen fixing potential of
different provenances of F. albida originated from Senegal and Burkina
Faso.
MATERIALS AND METHODS
Greenhouse experiment was carried out at Dakar (Bel-Air
experimental station) using local sandy soil (93% of soil). This soil has
a pH of 7.0, 1.9% total C and 0.25% total N. The soil was sieved (< 1
mm), homogeneized and placed into pots (20 Kg/pot) 30 cm in diameter.
TO each pot 1 g K,HPO, was added. Surface-sterilized seeds of Parkia
biglobosa and Faidherbia albida were germinated in petri dishes
containing soft agar 0.8% for 60 mn and 30 mn respectively. At 2 days
old, they were transplanted into the pots (1 seedling/pot).
There were eight treatments with five replicates : one non fixing
tree, i. e. Parkia biglobosa as reference crops and seven provenances of
Faidherbia albida from Senegal (Merina, Dangalma, Ndiongolor, Pire and
Kabrousse) and burkina Faso (Gomblora and Dem) inoculated with
Bradyrhizobium ORS 136 applied as liquid inoculum (10 ml/pot)
containing 10’ cells/ml. T O all p o t s , nitrogen fertilizer was then
applied at the rate of 0.2 g N/pot as a solution of (‘5N-l,),s0, with
10.01% atom excess. The pots were arranged randomly and watered SO
that the soil moisture was kept close to the field capacity.
At 6 months after transplantation into the pots, plants were
harvested. Plant and nodule dry weights were recorded. Nitrogen
content (%N) and %15N atom excess were determined by the IAEA
laboratory at Seibersdorf. Nitrogen fixation was estimated using both
isotope dilution and A-value methods.
5/01/94 - 2
Fin. Rpt
AIEA/6375/Rl/RB
RESULTS
P l a n t g r o w t h a n d nitrogen y i e l d
There were significant differences between F. albida provenances
in terms of %15N atom excess, dry matter (DM) and total nitrogen yield
(Table 1). For each plant part, the %15N atom excess of provenances of
Merina and Dangalma was higher than that of other trees including the
reference tree whereas the provenance of Kabrousse had the lowest
one. However, the provenance of Kabrousse seemed to yield more than
other provenances in terms of dry weight and total nitrogen of the
leaves and the stems even there was no significant difference between
the provenances in the roots and the whole plant in term of dry weight
and total nitrogen.
Nitrogen d e r i v e d f r o m f i x a t i o n
Whatever the method used, the proportion (%Ndfa) and amount
(Ndfa) of nitrogen derived from fixation are more important in the
provenance of Kabrousse than in the other provenances (Tables 2, 3, 4
and 5).
Refering to the isotope dilution technique (Tables 2 and 3), the
coefficient of variation was very high due to the variability between
the provenances : some of them (Merina and Dangalma) presented
negative values means that they did not fix nitrogen whereas the other
provenances showed a significant N, fixation even this fixation was
quite low, which was demonstrated in the previous study : the
pourcentage of nitrogen derived from fixation was -17.88%, -4.26%,
25.97%, 25.48%, 38.17%, 13.08 and 19.19% corresponding to an amount
of -0.06, -0.03, 0.26, 0.24, 0.44, 0.10 and 0.16 g N plant-’ for merina,
Dangalma, Ndiongolor, Pire, Kabrousse, Gomblora and Dem respectively.
Refering to A-value method (Tables 4 and 5), no negative value
was recorded. Nevertheless the provenances of Merina and Dangalma
fixed less nitrogen than the other provenances. The proportion of
nitrogen derived from fixation was 17.90%, 28.93%, 49,43%, 49.08%,
57.79%, 40.62% and 44.87% corresponding to an amount of 0.15, 0.21,
0.51, 0.44, 0.63, 0.31 and 0.36 g N plant-’ for Merina, Dangalma,
Ndiongolor, Pire, Kabrousse, Gomblora and Dem respectively. Similarly
to the isotope dilution technique, the coefficient of variation was high
due to the variability between the provenances.
5/01/94 - 3
Fin. Rpt
AIEA/6375/Rl/RB
Table 1. %15N atom excess, dry weight, nitrogen content and total nitrogen in different
plants parts of Parkia biglobosa and seven Faidherbia albida provenances cultivated in pots
containing 20 kg of non sterile soil.
____----------------------------.------------------------
Plant
T r e e
Dry weight
Total N
parts
species
Provenances*
%15NEX3
(g plant’)
%N
(g plant’)
- - - - - - - -
- - - - - -
-m-----m-
----
Leaves
P. biglobosa
0.691ab
1 3.35a
2.71b
F.albida
Merina (S)
0.776'
7.36'
2.87b
Dangalma (S)
0.745a
8.40bC
3.06b
Ndiongolor (S)
0.50gCd
12.18ab
2.75b
Pire (S)
0.523Cd
9.95ak
3.08b
Kabrousse (S)
0.415d
12.36ab
3.11b
Gomblora (BF)
0.596bC
6.66c
3.5ga
Dem (BF)
0.528Cd
7.80'
3.58*
c v (%)
14.60
24.10
8.70
24.50
Stems
P. biglobosa
0.64gbC
11.95bC
1.02c
0.12b
F. albida
Merina (S)
0.854'
7.77"
1.12bC
O.Ogb
Dangalma (S)
0.726"
9.10bC
1.18ak
O.llb
Ndiongolor (S)
0.49gCd
13.1 2abc
1.06bC
0.14ab
Pire (S)
0.496Cd
1 4.50ab
1.13bC
0.16ab
Kabrousse (S)
0.393d
18.43’
l.07bc
0.20a
Gomblora (BF)
0.581bcd
7.54c
1.37"
O.lOb
Dem (BF)
0.544bcd
9.80bC
1.28ab
0.13b
c v (%)
18.00
30.00
11.20
31.10
Roots
P. biglobosa
0.624ab
27.35"
1.28'
o.35a
F.albida
Merina (S)
0.729"
20.47"
1.73b
0.36a
Dangalma (S)
0.611ab
20.95a
1.76ab
0.37
Ndiongolor (S)
0.447bC
27.60a
1.8Pb
0.52"
Pire (S)
0.446bC
24.65"
1.66b
0.40a
Kabrousse (S)
o.404c
27.91a
1.84ab
0.50*
Gomblora (BF)
0.530bc
20.35a
2.06'
0.40a
Dem (BF)
0.511bC
19.51a
1.90ab
0.36"
c v (%)
19.70
34.10
9.60
34.80
Total
P. biglobosa
0.650abc
52.65a
1.67"
0.83a
F. albida
Merina (S)
0.780'
35.60a
1.91b
0-67
Dangalma (S)
0.700a
38.45a
2.00b
0.73a
Ndiongolor (S)
0.490de
52.90*
1 .90b
0.99*
Pire (S)
0.490d"
49.94"
1 .96b
0.87
Kabrousse (S)
0.410e
58.70"
2.01b
1.08*
Gomblora (BF)
0.570bcd
34.54*
2.34"
0.74"
Dem (BF)
o.530cde
37.11a
2.26'
0.77a
cv (%)
15.20
28.70
6.60
28.40
_____--_-------------------------------------------------
* : (BF) : Burkina Faso ; (S) : Senegal
For each plant part, values in the same column followed by the same letter do not differ
significantly at p = 0.05.
S/Ql/94-4
Fin. Rpt
AIEA/6375/Rl/RB
Table 2. Proportion of nitrogen derived from atmosphere (%Ndfa), fertilizer (%Ndff)
and soi1 (%Ndfs) in different plants parts of Parkia biglobosa and seven Faidherbia albida
provenances cultivated in pots containing 20 kg of non sterile soil using isotope dilution.
Plant
T r e e
parts
species
Provenances*
%Ndfa
%Ndff
%Ndfs
- - - - m----e- ----------_
- - - . - - -
- - - - - -
-m--m---
Leaves P. biglobosa
0.00
7.17ab
92.a3ab
F. albida
Merina (S)
-12.33"
8.05"
104.2aa
Dangalma (S)
- 7.87"
7.73"
100.14"
Ndiongolor (S)
26.34ab
5.2aCd
6a.3aCd
Pire (S)
24.31ab
5.43Cd
70.26Cd
Kabrousse (S)
39.a6a
4.31d
55.a3d
Gomblora (BF)
13.75b
6.1abC
a0.07bC
Dem (BF)
23.33ab
5.50Cd
71.1aCd
c v (%)
84.50
14.60
14.00
Stems
P. biglobosa
0.00
6.73bC
97.27bC
F. albida
Merina (S)
-31.65"
8.86"
122.79"
Dangalma (S)
-1 1.a6bC
7.53ab
104.33ab
Ndiongolor (S)
23.17a
5.1Td
71.65Cd
Pire (S)
23.64'
5.14Cd
71.22Cd
Kabrousse (S)
39.54a
4.07d
56.3gd
Gomblora (BF)
1 0.45ab
6.03bcd
a3.52bcd
Dem (BF)
16.15a
5.65bcd
7a.21Cd
c v (%)
174.70
la.00
17.70
Roots
P. biglobosa
0.00
6.47ab
95.53ab
F. albida
Merina (S)
-1 6.66b
7.56a
109.2ga
Dangalma (S)
2.05ab
6.34ab
91.61ab
Ndiongolor (S)
28.408
4.63bC
66.97bC
Pire (S)
28.498
4.63bC
66.aabC
Kabrousse (S)
35.13a
4.20'
60.67
Gomblora (BF)
15.00a
5.50bC
79.50bC
Dem (BF)
la.lia
5.30bC
76.5gbC
c v (%)
111.30
19.70
19.00
Total
P. biglobosa
0.00
6.7gabc
93.21ab
F. albida
Merina (S)
-17.aaC
a.16a
112.12a
Dangalma (S)
-4.26bC
7.20ab
9a.6aab
Ndiongolor (S)
25.97a
5.03d
69.00Cd
Pire (S)
25.4aa
C~06~
69.45cd
Kabrousse (S)
38.17a
4.6gd
57.63d
Gomblora (BF)
13.07ab
5.90bcd
81.03bC
Dem (BF)
19.1ga
5.4aCd
69.81Cd
c v (%)
100.60
15.10
15.50
_________---__-------------------------------------------
* : (BF) : Burkina Faso ; (S) : Senegal
For each plant part, values in the same column followed by the same letter do not differ
significantly at p = 0.05.
5/01/94 - 5
Fin. Rpt
AIEA/6375/Rl/RB
Table 3. Proportion of nitrogen derived from atmosphere (Ndfa), fettilizer (Ndff) and
soi1 (Ndfs) in different plants parts of Parkia biglobosa and seven Faidherbia aibida
provenances cultivated in pots containing 20 kg of non sterile soi1 using isotope dilution.
Plant
T r e e
Ndfa
Ndff
Ndfs
parts
species
Provenances*
(cl PL’)
(cl PL’)
kl PL’)
---_ - - - - - - -
- - - - - - -
- - - - - -
- - - - - -
Leaves P. biglobosa
0.00
0.030*
0.33*
F.albida
Merina (S)
-o.03c
0.020*
0.23*
Dangalma (S)
-o.02c
0.020*
0.26*
Ndiongolor (S)
O.Ogb
0.020*
0.23*
Pire (S)
0.07b
0.020*
0.22*
Kabrousse (S)
0.16'
0.020*
0.21*
Gomblora (BF)
0.03bC
0.01 o*
0.19*
Dem (BF)
0.06b
0.020*
0.15*
c v (%)
86.30
28.20
28.80
Stems
P. biglobosa
0.00
0.008*
0.1 la
F. albida
Merina (S)
-0.02d
0.008*
0.10*
Dangalma (S)
-O.OICd
0.008*
0.1 la
Ndiongolor (S)
0.03bC
0.007*
O.lOB
Pire (S)
0.04b
0.008*
0.1 la
Kabrousse (S)
0.08'
0.008"
0.10*
Gomblora (BF)
O.OiCd
0.006'
0.09*
Dem (BF)
0.02bcd
0.007*
o.oga
c v (%)
130.60
32.40
31.30
Fioots
P. biglobosa
0.00
0.020a
0.32a
F.a/bida
Merina (S)
-o.05c
0.030*
0.38a
Dangalma (S)
0.01 bc
0.020*
0.34*
Ndiongolor (S)
0.16ab
0.020*
0.34*
Pire (S)
0.12abc
0.020*
0.26'
Kabrousse (S)
0.20*
0.020*
0.29*
Gomblora (BF)
O.OGabC
0.020a
O-32*
Dem (BF)
0.08abc
0.020*
0.27
c v (%)
110.70
34.60
34.80
Total
P. biglobosa
0.00
0.05*
o.77a
F. albida
Merina (S)
-0.06'
0.05*
0.72*
Dangalma (S)
-o.03c
0.05*
0.71*
Ndiongolor (S)
0.26ab
0.05"
0.67"
Pire (S)
0.24ab
0,04*
0.59*
Kabrousse (S)
0.44*
0.05*
0.66*
Gomblora (BF)
O.lObC
0.04*
0.59*
Dem (BF)
0.16bC
0,04*
0.52*
c v (%)
95.40
34.10
29.40
* : (BF) : Burkina Faso ; (S) : Senegal
For each plant part, values in the same column followed by the same letter do not differ
significantly at p = 0.05.
5/01/94 - 6
Fin. Rpt
AIEA/6375/Ri/RB
Table 4. Proportion of nitrogen derived from atmosphere (%Ndfa), fertilizer (%Ndff)
and soi1 (%Ndfs) in different plants parts of Parkia biglobosa and seven Faidherbia albida
provenances cultivated in pots containing 20 kg of non sterile soil using A value method.
_____---------------------------------------------------
Plant
T r e e
parts
species
Provenances*
%Ndfa
%Ndff
%Ndfs
-__- - - - - - - -
---------_-
- - - - - -
- - - - - -
Leaves P. biglobosa
7.17ab
92.83”
F.albida
Merina (S)
20.38"
8.05'
71 .57b
Dangalma (S)
23.54"
7.73"
68.73b
Ndiongolor (S)
47.7Pb
5.28Cd
46.93Cd
Pire (S)
46.35ab
5.43Cd
48.22Cd
Kabrousse (S)
57.37a
4.31d
38.32d
Gomblora (BF)
38.86b
6.18bC
54.96’
Dem (BF)
45.65ab
5.50Cd
48.85Cd
c v (%)
23.00
14.60
13.10
Stems
P. biglobosa
6.73bC
93.27
F.aibida
Merina (S)
9.91C
8.86"
81.22ab
Dangalma (S)
23.45bC
7.53ab
69.02b
Ndiongolor (S)
47.43a
5.17Cd
47.40c
Pire (S)
47.74a
5.14Cd
47.1 lC
Kabrousse (S)
58.53a
4.07d
37.40"
Gomblora (BF)
38.72ab
6.03bcd
55.25'
Dem (BF)
42.62"
5.65bcd
51.73O
c v (%)
31.00
18.00
16.06
Roots
P. biglobosa
6.47ab
93.53”
F. albida
Merina (S)
23.42b
7.56a
69.02b
Dangalma (S)
39.8 Ila
6.34ab
57.85bC
Ndiongolor (S)
53.08a
4.63"
42.2gcd
Pire (S)
53.14a
4.63bC
42.23Cd
Kabrousse (S)
57.4ga
4.20'
38.31d
Gomblora (BF)
44.3aa
5.50bC
50.20Cd
Dem (BF)
46.33"
5.30bC
48.3Td
c v (%)
26.80
19.70
17.60
Total
P. biglobosa
93.21a
F. albida
Merina (S)
1 7.90c
73.94b
Dangalma (S)
28.93k
65.20'
Ndiongolor (S)
49.43a
45.54Cd
Pire (S)
49.08’
45.86Cd
Kabrousse (S)
57.7ga
38.01d
Gomblora (BF)
40.62ab
53.47
Dem (BF)
44.87"
49 .65cd
c v (%)
23.10
15.10
13.70
---------------------------------------------------------
* : (BF) : Burkina Faso ; (S) : Senegal
For each plant part, values in the same column followed by the same letter do not differ
significantly at p = 0.05.
5/01/94 - 7
F i n . Rpt
AIEA16375/Ri/RB
Table 5. Proportion of nitrogen derived from atmosphere (Ndfa), fertilizer (Ndff) and
soil (Ndfs) in different plants parts of Parkia biglobosa and seven Faidherbia albida
provenances cultivated in pots containing 20 kg of non sterile soil using A value method.
Plant
T r e e
Ndfa
Ndff
Ndfs
parts
species
Provenances*
(g PU
(9 pl.-‘)
(g Pl.“)
- - - - - - - - - - -
-a---------
---.---_
- - - - - -
- - - - - -
Leaves P. biglobosa
0.030a
0.33a
F.albida
Merina (S)
o.osd
0.020a
0.16b
Dangalma (S)
0.06Cd
0.020a
O.lab
Ndiongolor (S)
0.16b
0.020"
0.15b
Pire (S)
O.wb
0.020"
0.15b
Kabrousse (S)
0.22a
o.020a
0.14b
Gomblora (BF)
O.OSbCd
O.O1oa
0.13b
Dem (BF)
0.13bC
o.020a
0.14b
c v (%)
36.20
28.20
28.6
Stems
P. biglobosa
O.OOaa
F.albida
Merina (S)
O.OOaa
Dangalma (S)
O.OOaa
Ndiongolor (S)
0.007a
Pire (S)
O.OOaa
Kabrousse (S)
0.008"
Gomblora (BF)
0.006"
Dem (BF)
0.0078
c v (%)
52.00
32.40
31.00
Roots
P. biglobosa
o.020a
F. albida
Merina (S)
0.030"
Dangalma (S)
0.020a
Ndiongolor (S)
o.020a
Pire (S)
o.020a
Kabrousse (S)
0.020"
Gomblora (BF)
0.020"
Dem (BF)
o.020a
c v (%)
50.20
34.60
33.20
Total
P. biglobosa
0.050a
F.albida
Merina (S)
0.15"
0.050a
Dangalma (S)
0.21C
0.050a
Ndiongolor (S)
0.51ab
o.050a
Pire (S)
o.44abc
0.040a
Kabrousse (S)
0.63a
o.050a
Gomblora (BF)
0.31bC
o.040a
Dem (BF)
0.36abc
0.040a
c v (%)
42.60
34.10
27.40
__________----_------------------------------------------
* : (BF) : Burkina Faso ; (S) : Senegal
For each plant part, values in the same column followed by the same letter do not differ
significantly at p = 0.05.
5101184 - 8
F i n . Rpt
AlEA16375/Rl/RB
Nitrogen derlved f r o m f e r t i l i z e r and soi/
The proportion of nitrogen uptaken from fertilizer by ail F. albida
provenances was very low. It ranged from 4.69% in the provenance of
kabrousse to 8.16% in that of Merina. The proportion of nitrogen derived
from soi1 (%Ndfs) was higher in the provenances of Merina and
Dangalma than in the other provenances species. However, there was no
significant difference between the provenances in term of amount of
nitrogen derived from fixation and soil.
CONCLUSION
F. albida from Merina and Dangalma presented a very low N,
fixation. The provenance of Kabrousse was that with the higher NFP. It
should be selected for improving the soi1 fertlity SO far F. albida is
considered as a nitrogen fixing tree with an important agronomie
characteristic.
However, it is necessary to estimate the N, fixation in field
condition of the provenance of Kabrousse. This is the aim of the
renewalled contract (contract no. 6375/R2/RB) we have initiated.
We also keep in mind the another approach which involve grafting
the provenances of Merina and Dangalma onto that of Kabrousse or
grafting the provenance of Kabrousse onto Acacia seyal. This is also
initiated in the second renewal of the contract.
5/01/94 - 9
Fin. Rpt
AIEA/6375/Rl/RB