INSTITUT SENEGALAIS DE RECHERCHES AGRICOLES ...
INSTITUT SENEGALAIS DE RECHERCHES AGRICOLES
DIRECTION DES RECHERCHES SUR LES PRODUCTIONS FORESTIERES
CENTRE DE RESSOURCES MICROBIOLOGIQUES (MIRCEN)
SOIL AMELIORATION WITH NITROGEN-FIXING
ACACIA SPECIES
First progress r e p o r t o f I A E A contract no.6375/R2/RB
FIRST PROGRESS REPORT
CONTRACT NUMBER
6375/R2/RB
TITLE OF PROJECT
Soi1 amelioration with nitrogen fixing Acacia albida and Acacia seyal
INSTITUTE WHERE RESEARCH lS BEING CARRIED OUT
Institut Senegalais de Recherches Agricoles (ISRA)
CHIEF SCIENTIFIC INVESTIGATOR
Mamadou GUEYE
ADDITIONAL SCIENTIFIC STAFF :
Ibrahima NDOYE from Univ. C. A. DIOP, Dakar
Bernard DREYFUS from ORSTOM, Dakar
Simon BADJI from Eaux et Forets, Dakar
Pascal DANTHU from ISRA/DRPF
TIME PERIOD COVERED : June 1993 - June 1994
14/85/38- 1
1 s t R p t IAER 6375/RZ/RB
FIRST EXPERIMENT : FIELD EXPERIMENT
DESCRIPTION AND CARRYING OlJT THE EXPERIMENT
The experiment was carried out in the field on September 1993
and Will continue untill August 1994 with one provenance of Faidherbia
albida
originated from Kabrousse in the south of Senegal. Parkia
biglobosa was used as reference tree.
TREA TEMENTS
1) The Bradyrhizobium MAO 232 was used for inoculating the E
albida provenance in the nursery before transplanting into the field.
Three, 6, 9 and 12 months after transplantation, the fixed nitrogen Will
estimated.
2 ) The P. biglobosa served as reference tree.
NUMBER OF REPLICATES : 4
NUMEER OF PLOTS :
2 provenances x 4 harvests x 4 reps = 32 plots (then 32 subplots)
Plot size : 5 m x 3 m = 15 m2
Subplot size : 2 m x 1 m = 2 m2
Spacing : 1 m on the row ; 1 m between rows.
CALCULA TING THE NITROGEN REQUIREMENT
Amount of AS with 10.09% 15N a.e. Will be applied at 20 KgN/ha,
Le. 4 gN/subplot.
Total N requirement : 4 g N x 32 = 128 g N
Total AS requirement : 128 x 100/21.2 = 603.773 g AS
Vol. of solution needed : 500 ml/m2 x 2 x 32 = 32000 =32 I
14/05/30 -2
1st Rpt IAEA 6375/R2/RB
We shall use 40 I because of spillage. Thus, the required amount
of AS for 40 I is :
603.773 X 40/32 = 754.71625 g AS = 0.75 Kg AS.
Dilution
ml + m2 = 0.75 Kg AS
Ml = 132.3338 g/mole of AS 10.09% ’ 5N atom excess
M2 = 132 g/mole of ordinary AS
a’ = 10% ’ 5N a.e. desired in final dilution
a’1 = 10.09% ’ 5N a.e. of AS to be diluted
Then,
0.75 x 132.3338 x 10
ml =
__--------------------------------
(132.3338 x10.09) + (132.3338 - 132) x 10
ml = 0.74 Kg AS,
m2 = 0.01 Kg AS
Summary
ml = 0.74 Kg of As with 10.09% 15N a.e.
m2 = 0.01 Kg of ordinary AS
Total volume of solution : 40 I
Number of subplots : 32
Application rate : 1 Vsubplot
Remaining solution : 8 I
HARVEST
In December, the first samples were harvested. They Will be weighed
and sent to the agency for analysis.
14/85/38-3
1 s t R p t IAEA 6375/R2/RB
SECOND EXPERIMENT : GRAFTING EXPERIMENT
DESCRIPTION AND CARRYING OUT THE EXPERIMENT
This experiment was planed before, but not yet undertaken. The
aim of the experiment is to improve the biological nitrogen fixation in
Faidherbia albida with the grafting technique. It Will be carried out on
February 1994 and Will continue untill August 1994 with Faidherbia
albida. Based on the results obtained in the previous contract, the
provenances from Merina and Dagalma which fix less nitrogen Will be
grafted on the provenance from Kabrousse which fixes more nitrogen.
On the other hand, Merina and Dangalma Will be grafted on Acacia seyal
which has a high nitrogen fixing potential.
TREATMENTS
There Will be nine treatments :
T 1 : A. seyal alone inoculated with the strain ORS 1088
T 2 : Merina alone inoculated with the starin MAO 232
T 3 : Dangalma alone inoculated with the starin MAO 232
T 4 : Kabrousse alone inoculated with the strain MAO 232
T 5 : P. biglobosa alone without inoculation
T 6 : Merina grafted onto Kabrousse inoculated with MAO 232
T 7 : Dangalma grafted onto Kabrousse inoculated with MAO 232
T 8 : Merina grafted onto A. seyal inoculated with ORS 1088
T 9 : Dangalma grafted onto A. seyal inoculated with ORS 1088
NUMBER OF REPLICATES : 8
NUMBER OF POTS : 9 treatments x 8 reps = 72
CALCULA TING THE NITROGEN REQUIREMENT
Amount of AS with 10.09% “‘N a.e. Will be applied at
200 mg N/pots.
Total N requirement : 200 mg N x 72 = 14400 mg N
Total AS requirement : 14400 x lOW21.2 = 67924.5283 mg AS
14/85/30 - 4
1 s t R p t IREB 6375/R2/RB
Volume of solution needed : 50ml/pot x 72 = 3600 ml
We Will use 4000 ml because of spillage. Thus, the required amount of
AS for 4000 ml is :
67924.5283 x 4000/3600 = 75471.698 mg AS
Dilution
ml + m2 = 75471.698
Ml = 132.3338 g/mole of AS 10.09% ’ 5N a.e.
M2 = 132 g/mole of ordinary AS
a’ = 10% ’ 5N a.e. desired in final dilution
a’1 = 10.09% ’ 5N a.e. of AS to be diluted
Then,
75471.698 x 132.3338 xl 0
m
l
=
_____________-_-_------------
(132.3338 x 10.09) + (132.3338 _ 132) x 10
m l = 74611.98 mg AS
m2 = 859.72 mg AS
Summary
m l = 74.6 g of AS with 10.09% ’ 5N a.e.
m2 = 0.86 g of ordinaty AS
Total volume of solution : 500 ml
Number of pots : 72
Application rate : 50 ml/pot
Remaining solution : 400 ml.
TIME TABLE
15-28/2 : pregermination of seeds
1/3-30/4 : grafting and culture of plants into tubes
l-15/5 : acclimatization
16-‘20/5 : transplantation into pots, inoculation and application of
fertilizer
20/5-30/8 : transfert of pots in greenhouse ; end of the experiment.
14/85/3B - 5
1 s t R p t IAEA 6375/A2/RB
20 m
4
b
5m
b
.
A
D
C
B
’l.
C
B
D
A
’l.
D
B
A
c
’
f
A
C
B
D
’
t
Experimental design.
Harvest at 3 months (A), 6 months (B), 9 months (C) and
12 months (D) after transplantation
14/05/3B -6
1st Rpt IRER 6375/R2/RB
cjetailed exaerimental olot (size : 5m x 2m = 10 m2
0 Faidherbia albida
Parkia biglobosa
labelled subplot with 15N ammonium sulfate
(2m x lm = 2m2
14/05/30 -7
1st Rpt IAEA 6375/R2/RB
DIRECTION DES RECHERCHES SUR LES PRODUCTIONS FORESTIERES
CENTRE DE RESSOURCES MICROBIOLOGIQUES (MIRCEN)
SOIL AMELIORATION WITH NITROGEN-FIXING
ACACIA SPECIES
First progress r e p o r t o f I A E A contract no.6375/R2/RB
FIRST PROGRESS REPORT
CONTRACT NUMBER
6375/R2/RB
TITLE OF PROJECT
Soi1 amelioration with nitrogen fixing Acacia albida and Acacia seyal
INSTITUTE WHERE RESEARCH lS BEING CARRIED OUT
Institut Senegalais de Recherches Agricoles (ISRA)
CHIEF SCIENTIFIC INVESTIGATOR
Mamadou GUEYE
ADDITIONAL SCIENTIFIC STAFF :
Ibrahima NDOYE from Univ. C. A. DIOP, Dakar
Bernard DREYFUS from ORSTOM, Dakar
Simon BADJI from Eaux et Forets, Dakar
Pascal DANTHU from ISRA/DRPF
TIME PERIOD COVERED : June 1993 - June 1994
14/85/38- 1
1 s t R p t IAER 6375/RZ/RB
FIRST EXPERIMENT : FIELD EXPERIMENT
DESCRIPTION AND CARRYING OlJT THE EXPERIMENT
The experiment was carried out in the field on September 1993
and Will continue untill August 1994 with one provenance of Faidherbia
albida
originated from Kabrousse in the south of Senegal. Parkia
biglobosa was used as reference tree.
TREA TEMENTS
1) The Bradyrhizobium MAO 232 was used for inoculating the E
albida provenance in the nursery before transplanting into the field.
Three, 6, 9 and 12 months after transplantation, the fixed nitrogen Will
estimated.
2 ) The P. biglobosa served as reference tree.
NUMBER OF REPLICATES : 4
NUMEER OF PLOTS :
2 provenances x 4 harvests x 4 reps = 32 plots (then 32 subplots)
Plot size : 5 m x 3 m = 15 m2
Subplot size : 2 m x 1 m = 2 m2
Spacing : 1 m on the row ; 1 m between rows.
CALCULA TING THE NITROGEN REQUIREMENT
Amount of AS with 10.09% 15N a.e. Will be applied at 20 KgN/ha,
Le. 4 gN/subplot.
Total N requirement : 4 g N x 32 = 128 g N
Total AS requirement : 128 x 100/21.2 = 603.773 g AS
Vol. of solution needed : 500 ml/m2 x 2 x 32 = 32000 =32 I
14/05/30 -2
1st Rpt IAEA 6375/R2/RB
We shall use 40 I because of spillage. Thus, the required amount
of AS for 40 I is :
603.773 X 40/32 = 754.71625 g AS = 0.75 Kg AS.
Dilution
ml + m2 = 0.75 Kg AS
Ml = 132.3338 g/mole of AS 10.09% ’ 5N atom excess
M2 = 132 g/mole of ordinary AS
a’ = 10% ’ 5N a.e. desired in final dilution
a’1 = 10.09% ’ 5N a.e. of AS to be diluted
Then,
0.75 x 132.3338 x 10
ml =
__--------------------------------
(132.3338 x10.09) + (132.3338 - 132) x 10
ml = 0.74 Kg AS,
m2 = 0.01 Kg AS
Summary
ml = 0.74 Kg of As with 10.09% 15N a.e.
m2 = 0.01 Kg of ordinary AS
Total volume of solution : 40 I
Number of subplots : 32
Application rate : 1 Vsubplot
Remaining solution : 8 I
HARVEST
In December, the first samples were harvested. They Will be weighed
and sent to the agency for analysis.
14/85/38-3
1 s t R p t IAEA 6375/R2/RB
SECOND EXPERIMENT : GRAFTING EXPERIMENT
DESCRIPTION AND CARRYING OUT THE EXPERIMENT
This experiment was planed before, but not yet undertaken. The
aim of the experiment is to improve the biological nitrogen fixation in
Faidherbia albida with the grafting technique. It Will be carried out on
February 1994 and Will continue untill August 1994 with Faidherbia
albida. Based on the results obtained in the previous contract, the
provenances from Merina and Dagalma which fix less nitrogen Will be
grafted on the provenance from Kabrousse which fixes more nitrogen.
On the other hand, Merina and Dangalma Will be grafted on Acacia seyal
which has a high nitrogen fixing potential.
TREATMENTS
There Will be nine treatments :
T 1 : A. seyal alone inoculated with the strain ORS 1088
T 2 : Merina alone inoculated with the starin MAO 232
T 3 : Dangalma alone inoculated with the starin MAO 232
T 4 : Kabrousse alone inoculated with the strain MAO 232
T 5 : P. biglobosa alone without inoculation
T 6 : Merina grafted onto Kabrousse inoculated with MAO 232
T 7 : Dangalma grafted onto Kabrousse inoculated with MAO 232
T 8 : Merina grafted onto A. seyal inoculated with ORS 1088
T 9 : Dangalma grafted onto A. seyal inoculated with ORS 1088
NUMBER OF REPLICATES : 8
NUMBER OF POTS : 9 treatments x 8 reps = 72
CALCULA TING THE NITROGEN REQUIREMENT
Amount of AS with 10.09% “‘N a.e. Will be applied at
200 mg N/pots.
Total N requirement : 200 mg N x 72 = 14400 mg N
Total AS requirement : 14400 x lOW21.2 = 67924.5283 mg AS
14/85/30 - 4
1 s t R p t IREB 6375/R2/RB
Volume of solution needed : 50ml/pot x 72 = 3600 ml
We Will use 4000 ml because of spillage. Thus, the required amount of
AS for 4000 ml is :
67924.5283 x 4000/3600 = 75471.698 mg AS
Dilution
ml + m2 = 75471.698
Ml = 132.3338 g/mole of AS 10.09% ’ 5N a.e.
M2 = 132 g/mole of ordinary AS
a’ = 10% ’ 5N a.e. desired in final dilution
a’1 = 10.09% ’ 5N a.e. of AS to be diluted
Then,
75471.698 x 132.3338 xl 0
m
l
=
_____________-_-_------------
(132.3338 x 10.09) + (132.3338 _ 132) x 10
m l = 74611.98 mg AS
m2 = 859.72 mg AS
Summary
m l = 74.6 g of AS with 10.09% ’ 5N a.e.
m2 = 0.86 g of ordinaty AS
Total volume of solution : 500 ml
Number of pots : 72
Application rate : 50 ml/pot
Remaining solution : 400 ml.
TIME TABLE
15-28/2 : pregermination of seeds
1/3-30/4 : grafting and culture of plants into tubes
l-15/5 : acclimatization
16-‘20/5 : transplantation into pots, inoculation and application of
fertilizer
20/5-30/8 : transfert of pots in greenhouse ; end of the experiment.
14/85/3B - 5
1 s t R p t IAEA 6375/A2/RB
20 m
4
b
5m
b
.
A
D
C
B
’l.
C
B
D
A
’l.
D
B
A
c
’
f
A
C
B
D
’
t
Experimental design.
Harvest at 3 months (A), 6 months (B), 9 months (C) and
12 months (D) after transplantation
14/05/3B -6
1st Rpt IRER 6375/R2/RB
cjetailed exaerimental olot (size : 5m x 2m = 10 m2
0 Faidherbia albida
Parkia biglobosa
labelled subplot with 15N ammonium sulfate
(2m x lm = 2m2
14/05/30 -7
1st Rpt IAEA 6375/R2/RB